![]() Now what you want to do is take the samples and put them into your centrifuge and when you put them in, try to make sure that the hinges are always facing outwards, that way the pellet that forms will be on the same side as the hinge and it’ll be easy to see. Once you’ve added the 200 μL, put each tube on ice and leave it there for about 4 minutes. When you add the solution you may notice a little bit of a color change the solution originally is clear, but as you leave it and put it on the ice, it may become milky, so just pay attention to that color change when you see it. ![]() Then you’re going to add 200 μL of the protein precipitation solution to each one of the tubes. Because the protein precipitation solution can be potentially a little bit of an irritant, we can wear goggles at this point. This protein precipitation solution will take the proteins that are soluble, that are in the liquid right now, and force them to come out so that we can get rid of them when we actually want to extract the DNA. When you remove the samples from the heat block or the water bath, you may notice some color change from the oxidation, and of course at this point the nuclei, the chloroplasts, the mitochondria, they’ve been lysed by the solution, and we’re going to add protein precipitation solution. ![]() At this point your samples have been incubating for about 30 minutes: 15 minutes at 65 degrees and another 15 minutes at 37 with the RNase solution.
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